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Its longtime rival, the Canadian Pacific, has its rail system running through much of Canada as well as the upper Midwest including lines through Minnesota, Wisconsin, Iowa and tracks from Chicago across northern Illinois, through Iowa and Missouri to Kansas City.įreight Waves, a resource for the global freight industry, reports CP is North America's No. Primarily serving southern Canada, the railroad branches southward to New Orleans, including the former Illinois Central Gulf Railroad, running north and south through Illinois. In the meantime, both Canadian railroads are working to portray themselves as the ideal suitor for the Kansas City Southern.Ĭanadian National, commonly known as CN, is North America's fourth highest-earning railroad with nearly 20,000 route miles. STB's ongoing review of what is called a "voting trust" includes time for input from the public and other entities including Amtrak. This purchase still requires approval by the Surface Transportation Board, however. Then, a few weeks later, rival Canadian National made a $33.6 billion competing bid for the railroad, which was approved by KCS stock holders. In March, the CP offered $29 billion to buy the railroad. Railroads and rivalries at the forefront of the purchase is a century-old feud between Canada's largest railroad companies, the Canadian National and Canadian Pacific.īoth railroads have made pitches to buy the Kansas City Southern Railway in recent months. Mostly funded by federal and state dollars, the station will replace the current Amtrak station and ultimately become a central location for numerous regional transit services, along with Amtrak, Greyhound, and the Saluki Express. The timing of the possible acquisition also coincides with Carbondale's plans for a new $17.3 million transit hub in the downtown area. That's the reason I wanted to get involved," Henry told The Southern this week. "We just really can't afford a downgrade in service from Amtrak. His letter also said that the city feared the merger would mean "more traffic, more congestion and exacerbate an already frustrating situation." Others say the argument is part of a public relations battle and it is too soon to know the effect.Ĭarbondale Mayor John "Mike" Henry recently called for a "thorough review on how a CN-KCS deal would impact the already poor performance of Amtrak service in the region," in a letter dated July 20 and submitted to the Surface Transportation Board, the federal regulatory body reviewing the proposed merger. Some predict the proposed merger - if approved by federal regulators - will increase freight traffic through Southern Illinois and make passenger service to the area more unreliable. On May 21, the rail company entered into an agreement to combine with the Kansas City Southern railway. But one industry expert told The Southern it's still too early to tell what, if any, changes will take place.Ĭanadian National owns much of the railroads in the region including the north-south line from Chicago to New Orleans through communities including Centralia, Du Quoin and Carbondale. Alpha-heavy scenes see both consoles hit hard for short bursts, leading to temporary slowdown when magical attacks are unleashed. However, given the relatively low frame-rate it is surprising to see both consoles struggle to maintain a consistent level of performance, with PS4 and Xbox One exhibiting issues in different areas. The simplistic combat system and fairly relaxed timings allow for enjoyable gameplay, although latency and motion would certainly benefit from a faster 60fps update. Of course, this won't be surprising to fans of the series, where 30fps has been the standard since the original GameCube release. Dramatic camera movement and constant shifts in character positioning across the 3D plane highlight judder on both consoles as battles heat up, while controller inputs aren't quite as crisp as we'd like to see in a fighting game. The knock-on effect is that the fast and furious battles lack the silky fluidity we see in the likes of Street Fighter or Tekken. However, there's the sense that the developers have not attempted to push the envelope here: while resolution and effects work is boosted beyond last-gen standards, frame-rates remained capped at 30fps in-line with previous Naruto titles on Xbox 360 and PS3. In terms of performance, the move to current generation consoles gives Cyber Connect 2 more room to play with in terms of higher frame-rates as well as increased resolution. Lowering shadows to medium doesn't make much of a difference, leading us to believe the effect is somewhat bugged. Shadow quality at high (maximum settings) is also mostly a match for the PS4 game, though banding artefacts are often displayed across the environment, which are not present on console. Anti-aliasing options are limited to either FXAA or no AA at all, while motion blur and glare are selectable toggles. With PC, we're looking at a straight port of the console code with no tangible upgrade in graphical quality. Shadow quality is the only point of differentiation here, with these elements appearing darker and better defined across characters and dynamic objects on PS4, though this isn't always clearly visible in every scene. Paired with the 1080p pixel count, the rustic Japanese villages and colourful fighters stand out with much greater clarity compared to their last-gen counterparts and the visual experience is very, very close on both consoles - and indeed PC. The solid image quality makes the most of the core assets, while the use of hand-drawn textures gives the game a distinctive animated feel that remains faithful to the source material. The use of post-process anti-aliasing also results in a clean look to characters and environments, with only a few mild jaggies appearing around sub-pixel imagery and finer cel-shaded edges. Along with plentiful use of alpha transparencies for magical attacks, a depth of field effect and a crude frame-blending motion blur implementation add some cinematic flair to the proceedings while also enhancing the intensity of fast-paced scenes. In terms of the fundamentals, both PS4 and Xbox One resolve full 1080p resolution, combined with crisp texture work and a high level of anisotropic filtering that allows the art to remain clear at both long distances and oblique angles. On the whole, there's a sense that we're seeing something of a 'safe' upgrade over the last-gen entrants in the series, rather than a title that takes advantage of PS4 and Xbox One to their fullest extent. The target frame-rate is solidly in 30fps territory, while performance issues can distract on both consoles. It's a pretty comprehensive package but the situation is less impressive from a technical perspective, where developer Cyber Connect 2 could have done more to take advantage of the latest console hardware. Over 100 playable characters appear in this latest release, while enhancements include tag-team fighting, clothing/weapon damage and wall-running in addition to a variety of different gameplay modes. As a conclusion to the long-running Naruto saga, Ultimate Ninja Storm 4 delivers plenty of content for dedicated fans of the series. Results are the mean ± SD of three independent experiments (* p < 0.05, ** p < 0.01 *** p < 0.001). Quantization of viral DNA was performed using real-time quantitative PCR and using the standard curve generated in parallel and expressed as a concentration of ng/μl. (D) The viral DNA was extracted from Vero cells 24h post HSV-1 infection and NAABs treatment (0.06mg/mL), as described in the Materials and Methods. The cells were stained with crystal violet for plaques detection. After the incubation time, the inoculum was removed, and the monolayers were overlaid with DMEM containing 0.8% methylcellulose in the presence of Acyclovir at 0.5, 1, 10, and 20 μM. (C) Antiviral activity of Acyclovir: Vero cells infected with HSV-1 (F) and incubated for 1 h at 37 ☌. The EC 50 was obtained from nonlinear regression analysis of concentration-effect curves by the GraphPad Prism 6 program. (B) Antiviral activity of NAAB-496 and NAAB-503 was evaluated on the basis of plaques reduction assay in Vero cells infected with HSV-1 (F) and incubated in the presence of NAAB 496 and NAAB 503 at different concentrations (0.01, 0.05, 0.06, 0.08 and 0.1 mg/mL). The CC 50 was obtained from nonlinear regression analysis of concentration-effect curves by the GraphPad Prism 6 Demo program and expressed the means ± standard deviation of three independent experiments. The cell proliferation index (%) was determined at 72 hours of treatment on the basis of ATP levels using the ViaLight Plus Cell Proliferation and Cytotoxicity BioAssay Kit (Lonza Group Ltd., Basel, Switzerland). (A) The cytotoxicity effect of NAABs was evaluated on Vero cells in presence of different concentrations of NAABs compound (0.01, 0.05, 0.06, 0.08, 0.1, 0.15, 0.2, 0.4, 0.6, 0.8 and 1.6 mg/mL) separately. Viability assay and Antiviral activity of NAAB-496 and NAAB-503 in Vero cells. 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